Malaysian Tualang Honey Suppresses the Angiogenic Events in Endothelial Cells Induced by Vascular Endothelial Growth Factor

Anti-VEGF therapy has been used as the anti-angiogenic agent in cancer treatment. However, treatment often couples with severe complications. Complementary natural products that offer similar potency less side effects are sought alternatives. Study showed Malaysian Tualang Honey (MTH) can inhibit inflammation-induced vascular hyperpermeability vitro and vivo, but effect on angiogenesis process remains unclear. Thus, this study aims to determine of MTH. Effects MTH ranging from 0.3% 0.9% VEGF-induced human umbilical vein endothelial cells (HUVEC) was determined by proliferation, migration tube-formation assays. Matrix metalloproteinase-2 (MMP-2) secretion HUVEC VEGF production MCF7 response were also quantified using ELISA kits. Suramin, an angio-suppressive positive control. significantly suppressed proliferation (from 155% rate 54%); (~ 50% inhibition rate) (69% reduction) induced VEGF. These findings explained significant reduction (p < 0.05) MMP-2 39% suppression exhibited reduced (19% compared control) breast cells. Our suggest mainly targets cell through tube formation capacity, via prominent suppressing